Proficiency study

Accurate and internationally comparable human papillomavirus (HPV) DNA genotyping is essential for HPV vaccine research and for HPV surveillance. With the objective of improving and standardizing the quality of HPV laboratory services, the WHO established a global HPV laboratory network (HPV LabNet) in 2005. The main activities within the HPV LabNet were the harmonization and standardization of the laboratory procedures used for HPV vaccine research and HPV vaccination program impact monitoring by the development of internationally comparable quality assurance methods, international standards, and reference reagents, as well as a laboratory manual for vaccinology. In 2008, recombinant HPV DNA plasmids were used to establish international standards for HPV16 and HPV18 DNA, with an assigned potency in international units. That same year, WHO HPV LabNet conducted the first proficiency study that was open for participation from laboratories worldwide based on HPV DNA plasmids containing the genomes of 14 oncogenic and 2 benign HPV types. Since then, the proficiency study has been repeated, demonstrating that it is possible to perform continuous global studies based on plasmid DNA with unitage traceable to international standards, and that such studies can provide an overview of the status of HPV detection and typing methodologies worldwide.

The global HPV LabNet proficiency panels are designed for the genotyping needs in HPV vaccinology and the proficiency criteria are not intended for clinical HPV screening purposes, as the requirements for analytical sensitivity may be different.

To register for the HPV DNA proficiency panel and get more information contact EQUALIS: info@equalis.se.

Publications

2018

Continuing global improvement in human papillomavirus DNA genotyping services: The 2013 and 2014 HPV Labnet international proficiency studies. Eklund C, Forslund O, Walling KL, et al.

2014

Global improvement in genotyping of human papillomavirus DNA: The 2011 HPV Labnet International Proficiency Study. Eklund C, Forslund O, Wallin KL, et al.

2012

The 2010 global proficiency study of human papillomavirus genotyping in vaccinology. Eklund C, Forslund O, Wallin KL, et al.

2010

Global proficiency study on human papillomavirus genotyping. Eklund C, Zhou T, Dillner J, et al.

 

Reports

 

Technical Report on the Global HPV LabNet 2014 HPV DNA Genotyping Proficiency Panel

Technical Report on the Global HPV LabNet 2013 HPV DNA Genotyping Proficiency Panel

Technical Report on the HPV LabNet 2011 HPV DNA Genotyping Proficiency Panel

WHO HPV LabNet Report on HPV DNA Proficiency Panel 2010

WHO HPV LabNet Report on HPV DNA Proficiency Panel 2008