FAQ

SUBMITTING A NOVEL HPV TYPE

Can my “putative novel HPV type” be cloned in several plasmids?
Yes. Authors can use as many plasmids as needed.
Should I provide information about the bacteria and protocols used for cloning?
Not necessarily. The International HPV Reference Center only requires the plasmids containing the putative novel type and the whole DNA sequence for this type.
Should I provide the whole genomic sequence to the International HPV Reference Center?
Yes. The International HPV Reference Center will re-sequence the whole L1 gene for sequence confirmation, as well as some other random fragments.
Should I publish my sequence in GenBank?
In order to have an officially established HPV type, publishing its sequence in GenBank is not mandatory, but highly recommended. The International HPV Reference Center encourages all authors to promote open access.
How do I send the clones to the International Reference Center?
Authors can send plasmids by normal post, to the following address:

International HPV Reference Center
Carina Eklund
Institutionen för Laboratoriemedicin, avd. Patologi
F58 Karolinska Universitetssjukhuset Huddinge
141 86 Stockholm

There is no need for refrigeration.
When will I know if “my putative novel HPV type” is an officially established HPV type?
The submitting author will receive an email as soon as the International HPV Center has confirmed the sequence. At the same time, the novel type is posted as an officially established type in the International HPV Reference Center webpage.
I have blasted my HPV sequence and didn’t find another HPV with more than 90% similarity. Does that mean that my sequence corresponds to a putative novel HPV type?
Not always. Unfortunately, some sequences belonging to officially established HPV types are not public yet, as they have not been uploaded in GenBank. Therefore, the International HPV Reference Center, encourages all submitting authors, to send their sequence first (before proceding to cloning) to confirm novelty.
Should I provide the primers to the International HPV Reference Center for confirming my putative novel sequence?
It is not necessary, but much appreciated.

REQUEST A CLONE FOR ACADEMIC RESEARCH

Can I request several clones at a time?
Yes. But the International HPV Reference Center does not have resources to provide large numbers of clones for free. Requests for more than 10 clones will incur a processing charge.
Can I request clones to be used as positive controls in my experiments?
Yes
What is an MTA?
MTA stands for Material Transfer Agreement. It is an agreement form, needed to be signed by the requesting author. The International HPV Reference Center will send the clones to the requesting laboratory, once this MTA is signed.
Are all HPV types available for request?
In principle, yes. All HPV types whose owner gave permission to distribute, are available.

PROFICIENCY PANELS

What is the HPV proficiency panel?
The HPV proficiency panel consists of coded tubes with HPV DNA plasmids containing genomes of both oncogenic and benign HPV types. The panel composition is blinded to all laboratories participating in the global HPV proficiency study.
What is the global HPV proficiency study?
The global HPV proficiency study serves to promote the harmonization and standardization of laboratory procedures. After distributing HPV proficiency panels to participating laboratories and receiving the results, the International HPV Reference Center will evaluate their genotyping results, provide the results back to the participating laboratory and publish an aggregated overview of the status of HPV detection and typing services worldwide.
Please have a look at previous publications in the corresponding section.
Can I be part of the proficiency panel studies?
Yes. To register for the HPV DNA proficiency panel, write to: info@equalis.se.

Authors are welcome to write to hpvcenter@ki.se, if there is any question not answered here.